Klebsiella'nın tek tür ile kolonizasyonu enfeksiyon riskini artırıyor, yeni dizileme yöntemi ortaya koydu

Özet (abstract)

ABSTRACT Klebsiella pneumoniae and related species are a common cause of healthcare-associated infections. The gut is a major Klebsiella reservoir, and gut colonization is a risk factor for developing an extraintestinal Klebsiella infection. Patients can be colonized by multiple Klebsiella strains or even species in the gut simultaneously, and there is high concordance between the gut-colonizing and infection-causing strains. The detection and characterization of colonizing strains is critical for a better understanding of the progression to infection and for developing interventions for colonized patients. However, the association between mixed or mono-colonization and subsequent infection is unknown. In this study, we developed an amplicon-based sequencing method called wzi -Seq that enables the detection and quantification of Klebsiella strains from complex samples and mixtures using the conserved capsule gene wzi as a molecular barcode. This method is highly accurate and precise, with a sensitivity of 93% and specificity of 99.8% in mixtures containing as many as 58 unique wzi types, and 90.8% concordance with culture-based detection from archived rectal swab samples from an established case and control cohort. Adjudicating wzi -Seq with culture data, we determined that 63.2% (108/171) patients were mono-colonized with a single Klebsiella strain, while 36.8% (63/171) had mixed colonization with multiple Klebsiella strains. Controlling for patient variables in multivariate analysis, we determined that mono-colonization was significantly ( P = 0.034) associated with infection. Characterization of Klebsiella colonizing populations could improve the accuracy of assessing infection risk and enable targeted interventions to prevent these healthcare-associated infections. IMPORTANCE Klebsiella gut colonization is a major risk factor for the development of extraintestinal Klebsiella infections in hospitalized settings. However, it is unknown if patients are colonized by one or multiple strains of Klebsiella and how the population structure of Klebsiella in the gut impacts infection risk. Here, we describe the development of a novel technique, wzi -Seq, to detect and quantify multiple Klebsiella strains from complex samples using standard techniques and rapid DNA sequencing. We applied wzi -Seq to rectal swabs from a well-characterized patient cohort and found that Klebsiella colonization with a single strain was more prevalent than colonization with multiple strains. Furthermore, mono-colonized patients had a significantly higher risk of developing a Klebsiella infection than mixed-colonized patients. This work validates a new tool to study Klebsiella populations and reveals that population structure in the gut influences the risk of healthcare-associated infections.

Yazarların özeti; kaynağından alınmıştır. mSphere, 2026 · DOI ↗