Journal of Molecular Endocrinology· 2026Q2
Gestasyonel Diyabet ve Tip 1 Diyabetli Hamile Kadınlarda Farklı Dokuların Proteom Analizi
Proteome Analysis of Different Tissues in Pregnant Women with Gestational Diabetes and Type 1 Diabetes Mellitus
- 0atıf
- Q2SCImago
- 2026yıl
Kısa özet
Karbonik anhidraz 1 (CA1), gestasyonel diyabet (GDM) ve tip 1 diyabet (T1DM)li hamile kadınlarda omental yağ, plasenta ve iskelet kası dokularında tutarlı bir şekilde değişen tek proteindir; yağ ve plasenta dokularında GDM'de yukarı, T1DM'de ise aşağı yönlü düzenlenmiştir.
Yapay zekâ ile başlık ve abstract'tan üretildi; tam metin okunmaz.
Ana noktalar
- CA1, GDM ve T1DM'li hamile kadınlarda omental yağ, plasenta ve iskelet kası dokularında tutarlı bir şekilde değişen tek proteindir.
- CA1, GDM'de omental yağ ve plasenta dokularında yukarı yönlü, ancak T1DM'de bu dokularda aşağı yönlüdür.
- CA1, her iki GDM ve T1DM grubunda da iskelet kası dokusunda aşağı yönlüdür.
- Alfa-enolaz (ENOA), GDM'de plasenta ve iskelet kası dokularında yukarı yönlü, ancak T1DM'de aşağı yönlüdür.
- Tanımlanan tüm protein değişiklikleri istatistiksel olarak anlamlıydı (p < 0.05).
Yapay zekâ ile başlık ve abstract'tan üretildi; tam metin okunmaz.
Özet (abstract)
Abstract Background Gestational diabetes mellitus (GDM) and type 1 diabetes mellitus (T1DM) represent distinct hyperglycemic states during pregnancy. Although proteomic alterations have been described in plasma and placental tissues in GDM, no study has comprehensively compared proteomic changes across multiple relevant tissues in both GDM and T1DM within the same investigation. Methods Tissue samples of omental adipose tissue (OAT), placental tissue (PT), and skeletal muscle tissue (SMT) from pregnant women with GDM and T1DM were obtained during cesarean delivery. Protein extracts were analyzed using two-dimensional gel electrophoresis and difference gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry for protein identification. Protein–protein interaction analysis was performed using STRING. Selected proteins were further examined by western blotting. Results Carbonic anhydrase 1 (CA1) emerged as the only protein consistently altered in all three tissues. CA1 was upregulated in OAT and PT in GDM but downregulated in the same tissues in T1DM; in SMT, it was downregulated in both groups. Alpha-enolase (ENOA) demonstrated a similar inverse pattern, being upregulated in PT and SMT in GDM but downregulated in T1DM. All of these changes identified by 2DE analysis were statistically significant (p < 0.05). Conclusion The findings of this study demonstrated that CA1 expression was significantly upregulated in OAT and PT, and ENOA expression was upregulated in PT and SMT. These alterations may reflect differences in the metabolic and hyperglycemic environments of the two conditions and may represent candidate tissue-level markers. Further studies are required to determine their functional and clinical significance.
Yazarların özeti; kaynağından alınmıştır. Journal of Molecular Endocrinology, 2026 · DOI ↗
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