Epiberberine ve Palmatin, Src/Syk Sinyal Yolunu İçeren Konak İnflamatuar Yanıtlarını Modüle Ederek Staphylococcus aureus Kaynaklı Akut Akciğer Hasarını Hafifletir

Özet (abstract)

Abstract Acute lung injury (ALI) caused by bacterial infection is largely driven by dysregulated host inflammatory responses, highlighting the need for host-directed therapeutic strategies. In this study, we investigated alkaloids derived from Coptis chinensis , focusing on epiberberine and palmatine, in both in vivo and in vitro models of Staphylococcus aureus -induced ALI. Unlike conventional endotoxin-based models, a live bacterial infection system was employed to better capture the complexity of host-pathogen interactions. Treatment with epiberberine and palmatine markedly attenuated lung injury in mice, as evidenced by reduced inflammatory cell infiltration and alleviated histopathological damage, accompanied by decreased production of pro-inflammatory mediators (TNF-α, IL-1β, and RANTES) and differential regulation of IL-10. Consistent anti-inflammatory effects were observed in peritoneal macrophages following bacterial stimulation. Integrated transcriptomic profiling and network analysis identified Src and Syk as putative upstream regulators associated with infection-induced inflammatory responses. Both compounds were observed to suppress Src/Syk phosphorylation and downstream MAPK signaling, while pharmacological inhibition of Src or Syk produced comparable effects without additive responses. Collectively, these findings suggest that epiberberine and palmatine alleviate bacterial pneumonia-associated lung injury by modulating host inflammatory responses, potentially involving Src/Syk-associated signaling pathways, supporting their potential as candidates for host-directed therapy.

Yazarların özeti; kaynağından alınmıştır. Inflammation, 2026 · DOI ↗