Sinaptik vezikül ayrılması düşük frekanslı depresyona neden olur

Özet (abstract)

Abstract Synaptic depression is often interpreted as reflecting depletion of the readily releasable pool (RRP) following exocytosis. Such a mechanism predicts little or no depression at low stimulation frequency, as RRP replenishment should then offset the loss of vesicles by exocytosis. Nevertheless, in several types of mammalian central synapses, repetitive presynaptic stimulation at low frequency (< 5 Hz) elicits synaptic depression (low frequency depression, or LFD). In the present work we count the number of synaptic vesicles released at individual active zones to study the RRP and its replenishment during LFD. Contrary to depletion models of synaptic depression, we find that LFD does not depend on previous SV consumption. We find that LFD displays a long recovery time course (tens of seconds) when challenged by isolated stimulations but is immediately reversed by a high frequency train. We suggest that LFD results from undocking, a shift between two classes of synaptic vesicles organized sequentially inside the RRP (replacement vs. docked vesicles) in favor of the upstream (replacement) state. While undocking is apparent hundreds of milliseconds after a stimulation, calcium dependent docking takes only a couple of milliseconds, explaining the fast LFD recovery when stimulating at high frequency. Consistent with the undocking model, we find that double presynaptic stimulations alleviate LFD as they favor vesicular docking and RRP replenishment. Finally, we expand our model to explain how stimulation frequency shapes short-term synaptic depression, changing from depression at low frequency to a facilitation-depression sequence at medium or high frequency trains.

Yazarların özeti; kaynağından alınmıştır. eLife, 2026 · DOI ↗