Biomedical Optics Express· 2026Q1
Two-module upgrade for 3D, high-resolution microscopy DMD patterned excitation and light-field detection
- 1citations
- Q1SCImago
- 2026year
Short summary
A modular microscope upgrade integrates patterned illumination (SIM) with light-field detection (FLFM) for high-resolution 3D biological imaging, achieving up to 1.4x lateral resolution improvement in organoid samples.
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Key points
- A new modular upgrade integrates SIM and FLFM for high-resolution 3D biological imaging.
- The system uses a DMD for patterned illumination and a light-field detection path.
- MAP-SIM and ML-SIM reconstruction methods were employed for enhanced resolution and background suppression.
- Murine intestinal organoids showed up to 1.4x lateral resolution improvement with SIM prior to FLFM.
- FLFM acquisition significantly reduced photobleaching compared to conventional z-stack imaging.
AI-generated from the title and abstract; the full text is not read.
Abstract
We present a modular upgrade to conventional widefield microscopy that integrates Fourier light field microscopy (FLFM) with structured illumination microscopy (SIM) for high-resolution volumetric biological imaging. The system combines a digital micromirror device (DMD)-based patterned illumination module with a light field detection path added to a commercial microscope, enabling flexible switching between widefield and FLFM modalities. By incorporating both analytical (MAP-SIM) and machine-learning-based (ML-SIM) reconstruction methods, we demonstrate significant resolution enhancement and background suppression in both 2D and 3D imaging. Experimental validation on murine intestinal organoids shows that applying SIM prior to FLFM reconstruction yields up to 1.4× lateral resolution improvement while preserving volumetric information. Additionally, FLFM acquisition drastically reduces photobleaching compared to conventional z-stack imaging. The proposed setup provides a cost-effective and versatile platform for multi-color, 3D imaging, with potential applications in microscopy education, or live-cell imaging.
The authors' abstract, as published at the source. Biomedical Optics Express, 2026 · DOI ↗
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BiophysicsBiochemistry, Genetics and Molecular Biology