RiboTRAP-seq, Caenorhabditis elegans'de bağışıklık ve metabolik düzenlemede ön ve arka bağırsağın uzamsal olarak farklılaşmış fonksiyonlarını tanımlar

Özet (abstract)

Abstract The intestine integrates food-derived cues to coordinate organismal physiology, yet the molecular specialization of discrete regions along the intestinal epithelium remains unclear. Here, we generate cell type-specific translatomes of the Caenorhabditis elegans intestine during fasting and refeeding using discrete promoters for the anterior quartet of intestinal cells (INT1) and the remaining 8 pairs of intestinal cells (INT2-9). We found that the anterior-most INT1 cells are a translationally distinct intestinal sub-compartment that is particularly enriched for immune-and stress-response genes. Functional assays using novel INT1-specific genes emerging from this study reveal that these specialized cells play a previously unappreciated role in pathogen avoidance and organismal survival. A second critical function of INT1 cells is their role in sensing and responding to the contents of the gut lumen. We show that luminal pyruvate is the key signal linking bacterial nutrients in the gut, to secretion of the gut insulin antagonist INS-7. These findings establish INT1 as sentinel enteroendocrine cells that integrate metabolic and immune cues to couple food status with immune and endocrine responses. Our studies also provide a rich resource for dissecting segment-specific intestinal biology, an overlooked and fertile area for future research.

Yazarların özeti; kaynağından alınmıştır. eLife, 2026 · DOI ↗