The Journal of Cell Biology· 2026Q1
ARF-1 coordinates cargo sorting at FERARI endosomal recycling hubs
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- 2026year
Short summary
The small GTPase ARF-1 is identified as essential for loading cargo into recycling vesicles at FERARI-dependent endosomal kiss-and-run sites.
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Key points
- ARF-1 is identified as an essential regulator of cargo loading at FERARI-dependent endosomal kiss-and-run (KAR) sites.
- ARF-1's cycling between active and inactive states is required for efficient cargo capture into recycling vesicles.
- ARF-1 activity governs the temporal association of cargo with adaptor proteins, controlling selective vesicle uptake.
- This work uncovers a new layer of regulation in endosomal recycling and highlights ARF-Rab GTPase interplay.
AI-generated from the title and abstract; the full text is not read.
Abstract
Endosomal pathways are central to cellular organization, compartmentalization, and intercellular communication. Independent of the endocytic entry route, sorting endosomes function as critical junctions at which membrane proteins are sorted for recycling or degradation. The multicomponent tether FERARI orchestrates recycling by coordinating Rab GTPase activity with vesicle fusion and fission through a kiss-and-run (KAR) mechanism. Here, we identify the small GTPase ARF-1 as an essential regulator of cargo loading at FERARI-dependent endosomal KAR sites. Cycling of ARF-1 between its active and inactive states is required for efficient cargo capture into recycling vesicles. Our findings support a model in which cycling ARF-1 activity governs the temporal association of cargo with adaptor proteins and complexes, thereby controlling selective uptake into vesicles destined for the plasma membrane. These results uncover a previously unappreciated layer of regulation of endosomal recycling and highlight the interplay between ARF and Rab family GTPases in this process.
The authors' abstract, as published at the source. The Journal of Cell Biology, 2026 · DOI ↗
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Field: Cell Biology
Cell BiologyBiochemistry, Genetics and Molecular Biology