Stem Cell Research & Therapy· 2017Q1
Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study
- 409citations
- Q1SCImago
- 2017year
Short summary
A pilot study found autologous transplantation of mobilized dental pulp stem cells (MDPSCs) with G-CSF to be safe and efficacious for complete pulp regeneration in teeth with irreversible pulpitis.
AI-generated from the title and abstract; the full text is not read.
Key points
- Autologous MDPSCs with G-CSF were safely transplanted into pulpectomized teeth in five patients with irreversible pulpitis.
- No adverse events or toxicity were reported during the 24-week follow-up.
- Electric pulp testing showed positive responses at 4 weeks post-transplantation.
- MRI indicated regenerated tissue signal intensity comparable to normal dental pulp by 24 weeks.
- Functional dentin formation was observed in three of the five patients.
AI-generated from the title and abstract; the full text is not read.
Abstract
BACKGROUND: Experiments have previously demonstrated the therapeutic potential of mobilized dental pulp stem cells (MDPSCs) for complete pulp regeneration. The aim of the present pilot clinical study is to assess the safety, potential efficacy, and feasibility of autologous transplantation of MDPSCs in pulpectomized teeth. METHODS: Five patients with irreversible pulpitis were enrolled and monitored for up to 24 weeks following MDPSC transplantation. The MDPSCs were isolated from discarded teeth and expanded based on good manufacturing practice (GMP). The quality of the MDPSCs at passages 9 or 10 was ascertained by karyotype analyses. The MDPSCs were transplanted with granulocyte colony-stimulating factor (G-CSF) in atelocollagen into pulpectomized teeth. RESULTS: The clinical and laboratory evaluations demonstrated no adverse events or toxicity. The electric pulp test (EPT) of the pulp at 4 weeks demonstrated a robust positive response. The signal intensity of magnetic resonance imaging (MRI) of the regenerated tissue in the root canal after 24 weeks was similar to that of normal dental pulp in the untreated control. Finally, cone beam computed tomography demonstrated functional dentin formation in three of the five patients. CONCLUSIONS: Human MDPSCs are safe and efficacious for complete pulp regeneration in humans in this pilot clinical study.
The authors' abstract, as published at the source. Stem Cell Research & Therapy, 2017 · DOI ↗
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Field: Oral Surgery
Oral SurgeryDentistry