Chemical & Biomedical Imaging· 2026Q1
Millimeter-Scale Wide-Field Mid-Infrared Photothermal Imaging Enabled by a Broadly Tunable Picosecond Optical Parametric Oscillator
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- Q1SCImago
- 2026year
Short summary
A new mid-infrared photothermal (MIP) imaging platform achieves millimeter-scale fields of view (FOV) with submicron resolution, overcoming previous limitations of FOV size and spectral range.
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Key points
- Developed a wide-field fluorescence-detected MIP (F-MIP) platform using a picosecond optical parametric oscillator (OPO).
- Achieved millimeter-scale fields of view (∼1 mm) with submicron resolution (0.5–5 μm beads).
- Enabled wide-field MIP imaging across a broad spectral range, including below 900 cm–1 for the first time.
- Demonstrated chemically resolved mapping of tuberculosis-infected tissue sections over millimeter-scale areas.
AI-generated from the title and abstract; the full text is not read.
Abstract
Abstract Wide-field mid-infrared photothermal (MIP) imaging enables chemically specific microscopy with submicron spatial resolution but remains fundamentally limited by the trade-off between field of view, mid-infrared pulse energy, and spectral tunability. As a result, current wide-field implementations are typically restricted to fields of view (FOVs) below 200 μm and to either the fingerprint or high-wavenumber spectral regions. Here, we present a wide-field fluorescence-detected MIP (F-MIP) platform driven by a commercial picosecond optical parametric oscillator (OPO) delivering up to 360 μJ across 625–4347 cm–1, enabling millimeter-scale imaging in the high-wavenumber region (∼1 mm FOV, 0.5–5 μm polystyrene beads) and, to our knowledge, the first wide-field MIP imaging below 900 cm–1. Large-area mosaic imaging of tuberculosis-infected tissue sections demonstrates chemically resolved mapping over millimeter-scale biomedical specimens, establishing OPO-driven F-MIP as a scalable platform for high-throughput vibrational imaging.
The authors' abstract, as published at the source. Chemical & Biomedical Imaging, 2026 · DOI ↗
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BiophysicsBiochemistry, Genetics and Molecular Biology