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BMC Biotechnology· 2026Q2

Extraction, physicochemical, thermal, and rheological characterization of gelatin from milkfish (Chanos chanos) skin using different pretreatment and extraction methods

Noora Barzkar, Vanessa Precious Lasiuan, Adrian Haqimi Mu’iz Bin Jamain

Short summary

Ultrasound-assisted extraction combined with sulfuric and acetic acid pretreatment yielded the highest milkfish skin gelatin (42.63% ± 1.63%), with improved thermal (melting 33.30°C, gelling 19.70°C) and rheological properties (viscosity 11.92 cP, gel strength 219.57 g) compared to conventional methods.

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Key points

  • Ultrasound-assisted extraction with sulfuric and acetic acid pretreatment yielded a maximum gelatin yield of 42.63% ± 1.63% from milkfish skin.
  • This method improved gelatin's thermal properties, with melting at 33.30°C and gelling at 19.70°C.
  • Gelatin produced via ultrasound and acetic acid pretreatment showed enhanced viscosity (11.92 cP) and gel strength (219.57 g).
  • Color analysis indicated that natural pigmentation was retained, with ΔE* values between 55.37 and 59.19.

AI-generated from the title and abstract; the full text is not read.

Abstract

Abstract The study investigated the extraction and characterization of gelatin from milkfish skin ( Chanos chanos ) using different pretreatment and extraction methods. The effects of acetic acid, combined sulfuric acid-acetic acid pretreatments, ultrasound-assisted extraction, and optimized multi-step extraction were evaluated. Extraction conditions significantly affected gelatin yield and functional properties. The highest yield (42.63 ± 1.63%) was obtained using ultrasound-assisted extraction combined with sulfuric acid and acetic acid pretreatment. Ultrasound treatment also improved the thermal and rheological properties of gelatin, resulting in higher melting (33.30 °C) and gelling temperatures (19.70 °C). Gelatin produced using acetic acid pretreatment and ultrasound exhibited greater viscosity (11.92 cP) and gel strength (219.57 g) than gelatin obtained through conventional extraction methods. Color analysis showed ΔE* values ranging from 55.37 to 59.19, indicating retention of the natural gelatin pigmentation. Overall, ultrasound-assisted extraction combined with appropriate acid pretreatment enhanced gelatin yield and improved its physicochemical, thermal, and rheological characteristics.

The authors' abstract, as published at the source. BMC Biotechnology, 2026 · DOI ↗

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Field: Biomaterials

BiomaterialsMaterials Science