Organic Letters· 2026Q1
Functional Characterization of Cross-Link Formation in Atropopeptide P450 Synthases
- 0citations
- Q1SCImago
- 2026year
Short summary
Researchers functionally characterized three unknown atropopeptide P450 synthases (KutB, KutC, and NteB), with KutC and NteB providing the first in vitro analysis of these enzymes and revealing NteB's ability to generate both bridge-above and bridge-below atropisomers.
AI-generated from the title and abstract; the full text is not read.
Key points
- KutC and NteB are the first atropopeptide P450 synthases to be characterized in vitro.
- NteB generates both bridge-above and bridge-below atropisomers.
- Docking models and mutational analysis suggest key residues for substrate recognition in KutB, KutC, and NteB.
- The study elucidates the biosynthesis of atropopeptides, a class of ribosomally synthesized and post-translationally modified peptides.
AI-generated from the title and abstract; the full text is not read.
Abstract
Abstract Biaryl cross-link-containing peptides make up an emerging class of ribosomally synthesized and post-translationally modified peptides (RiPPs). The C–C, C–N, and C–O cross-links in atropopeptides are installed by atropopeptide P450 synthases. In this work, we characterized three unknown atropopeptide P450 synthases (KutB, KutC, and NteB). Functional characterization of KutC and NteB provided the first in vitro analysis of atropopeptide P450 synthases and revealed that NteB generates both bridge-above and bridge-below atropisomers. Furthermore, docking models of KutB, KutC, and NteB, together with mutational analysis, suggested putative substrate recognition residues. This study provides important insights into the biosynthesis of atropopeptides.
The authors' abstract, as published at the source. Organic Letters, 2026 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Pharmacology (Medicine)
PharmacologyMedicine