Cell stem cell· 2026Q1
EPCR expression marks CD150neg deeply dormant long-term hematopoietic stem cells
- 1citations
- Q1SCImago
- 2026year
Short summary
A novel subset of long-term hematopoietic stem cells (LT-HSCs), termed LT-HSC2, identified by endothelial protein C receptor (EPCR) expression and absence of CD150, exhibits deeper dormancy and reduced cell cycle activation under inflammatory stress compared to conventional LT-HSCs.
AI-generated from the title and abstract; the full text is not read.
Key points
- A novel LT-HSC subset (LT-HSC2) is defined by EPCR expression and CD150 negativity.
- LT-HSC2s are more deeply dormant than conventional EPCR+CD150+ LT-HSC1s.
- LT-HSC2s show reduced cell cycle activation in response to inflammatory stress.
- EPCR and CD150 markers can resolve early lineage divergence trajectories.
AI-generated from the title and abstract; the full text is not read.
Abstract
Cell surface expression of the Slam family member CD150 is widely used for the prospective isolation of multipotent long-term hematopoietic stem cells (LT-HSCs). Here, we identify a bona fide endothelial protein C receptor (EPCR) + CD150 − HSC subset, termed LT-HSC2, that sustains serial multi-lineage reconstitution. Long-term label-retaining assays reveal that LT-HSC2s are more deeply dormant than conventional EPCR + CD150 + LT-HSC1s, exhibit reduced cell cycle activation in response to inflammatory stress, and preserve stemness for prolonged periods in vivo . Moreover, combining EPCR with established HSC and multipotent progenitor (MPP) markers resolves the earliest stages of lineage divergence toward megakaryocytic/erythroid-biased CD150 + and lymphoid-biased CD150 − trajectories. Thus, EPCR expression in HSCs and MPPs is associated with increased stemness, whereas CD150 expression does not encompass the full spectrum of long-term repopulating HSCs. Our findings establish EPCR + CD150 − LT-HSC2s as a distinct, exceptionally dormant stem-cell subset and reveal an additional layer of HSC heterogeneity governing self-renewal, decreased stress sensitvity. and earliest lineage priming.
The authors' abstract, as published at the source. Cell stem cell, 2026 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Hematology
HematologyMedicine