Yapay Zeka Destekli Birmeren Floresan Prob Aracılığıyla Gıda Tazeliği İzleme İçin Hidrojen Sülfür İçin Oransal Algılama

Özet (abstract)

The development of robust, portable sensors for the real-time monitoring of hydrogen sulfide (H 2 S), a key volatile biomarker of protein degradation, is essential for evaluating food spoilage. Herein, we report the rational design of CMR-DCN, a coumarin-based donor-acceptor fluorescent probe featuring distinct aggregation-induced emission (AIE) characteristics. Overcoming the traditional aggregation-caused quenching effect, CMR-DCN maintains intense solid-state fluorescence, making it highly suitable for practical device fabrication. The sensing mechanism relies on the specific nucleophilic addition of H 2 S, which interrupts the probe's intramolecular charge transfer (ICT) process. This interaction triggers a pronounced hypsochromic emission shift from 585 nm to 480 nm (99 vol% water fraction), providing a highly selective ratiometric response with a low limit of detection of 0.7 μM. To translate this molecular probe into a practical sensing device, we fabricated “smart” test strips integrated with a smartphone-based colorimetric (B/R channel) platform for portable, instrument-free analysis. This integrated platform successfully quantified gaseous H 2 S and visually monitored real-time spoilage in shrimp and beef samples with high reliability. Furthermore, because its dense AIE nanoaggregate state effectively suppresses cross-reactivity with oxidative interferents such as hypochlorous acid (HOCl), CMR-DCN was successfully deployed for the highly specific ratiometric bioimaging of exogenous and endogenous H 2 S in living cells. This work presents a robust molecular engineering strategy for AIE-active materials and delivers a versatile sensing platform for intelligent food packaging and advanced biological detection.

Yazarların özeti; kaynağından alınmıştır. Microchemical Journal, 2026 · DOI ↗