Viruses· 2026Q1
Functional Evaluation of Neutralizing Antibodies Against Foot-and-Mouth Disease Virus Serotype O Using a Luciferase-Based Surrogate Neutralization Assay
- 0citations
- Q1SCImago
- 2026year
Short summary
A new luciferase-based assay accurately measures neutralizing antibody activity against Foot-and-Mouth Disease Virus (FMDV) serotype O, correlating strongly (R²=0.9068) with traditional methods but avoiding live virus handling.
AI-generated from the title and abstract; the full text is not read.
Key points
- A luciferase-based surrogate neutralization assay was established for FMDV serotype O.
- The assay uses HiBiT-tagged VLP preparations and LgBiT-expressing cells, measuring luminescent signal reduction.
- Surrogate assay results strongly correlated with conventional virus neutralization tests (R² = 0.9068).
- VLPs used in the assay showed stable expression, assembly, and morphology similar to FMDV.
AI-generated from the title and abstract; the full text is not read.
Abstract
Foot-and-mouth disease (FMD) is a highly contagious viral disease that seriously threatens livestock health. Protective immunity induced by vaccination is primarily associated with the generation of neutralizing antibodies; however, conventional virus neutralization tests are time-consuming and require the handling of live virus, limiting their suitability for rapid and repeated evaluation in routine settings. In this study, we established a surrogate neutralization assay for the functional evaluation of neutralizing antibody activity against FMD virus (FMDV) serotype O. The assay measures changes in luciferase-based luminescent signals generated following exposure of LgBiT-expressing cells to HiBiT-tagged virus-like particle (VLP) preparations. FMDV serotype O-derived VLPs exhibited stable capsid protein expression, assembly characteristics, and morphological integrity similar to those of virus particles, as confirmed by sucrose gradient fractionation and electron microscopy. In the presence of neutralizing antibodies, a reduction in luminescent signals was observed, enabling functional discrimination of antibody activity. The surrogate assay results showed a strong correlation with those of the conventional virus neutralization test for FMDV serotype O (R2 = 0.9068). This study demonstrates the feasibility of the surrogate neutralization assay for assessing the functional activity of neutralizing antibodies and its potential applicability to vaccine immunogenicity assessment and functional antibody analysis.
The authors' abstract, as published at the source. Viruses, 2026 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Agronomy and Crop Science
Agronomy and Crop ScienceAgricultural and Biological Sciences