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Chemical Reviews· 2013Q1· Review

Protein Analysis by Shotgun/Bottom-up Proteomics

Yaoyang Zhang, Bryan R. Fonslow, Bing Shan, Moon‐Chang Baek et al.

Short summary

Shotgun/bottom-up proteomics enables comprehensive protein identification and quantification from complex biological samples by digesting proteins into peptides, separating them via liquid chromatography, and analyzing them with mass spectrometry.

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Key points

  • Shotgun/bottom-up proteomics identifies proteins by analyzing peptides generated from enzymatic digestion.
  • Liquid chromatography is used to separate these peptides prior to mass spectrometry analysis.
  • Tandem mass spectrometry (MS/MS) is the core technology for peptide identification.
  • The technique allows for high-throughput analysis of complex protein mixtures.

AI-generated from the title and abstract; the full text is not read.

Abstract

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTProtein Analysis by Shotgun/Bottom-up ProteomicsYaoyang Zhang†, Bryan R. Fonslow†, Bing Shan†, Moon-Chang Baek†‡, and John R. Yates, III*†View Author Information† Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037, United States ‡ Department of Molecular Medicine, Cell and Matrix Biology Research Institute, School of Medicine, Kyungpook National University, Daegu 700-422, Republic of Korea*Phone: +1-858-784-8862. Fax: +1-858-784-8883. E-mail: [email protected]Cite this: Chem. Rev. 2013, 113, 4, 2343–2394Publication Date (Web):February 26, 2013Publication History Received21 August 2012Published online26 February 2013Published inissue 10 April 2013https://pubs.acs.org/doi/10.1021/cr3003533https://doi.org/10.1021/cr3003533review-articleACS PublicationsCopyright © 2013 American Chemical SocietyRequest reuse permissionsArticle Views28754Altmetric-Citations1079LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Ions,Monomers,Peptides and proteins,Post-translational modification,Proteomics Get e-Alerts

The authors' abstract, as published at the source. Chemical Reviews, 2013 · DOI ↗

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Field: Spectroscopy

SpectroscopyChemistry