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Journal of Clinical Microbiology· 2026Q1

Advanced molecular poliovirus diagnostics across multiple real-time PCR platforms with locked nucleic acids and primer overhangs

Nancy Gerloff, Hong Sun, Brandon Short, Eric Harvey et al.

Short summary

Five new real-time RT-PCR assays using locked nucleic acids (LNA) and primer overhangs achieve 100% sensitivity for detecting extinct wild-type 3 poliovirus and circulating wild-type 1 poliovirus, and differentiate vaccine-derived strains.

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Field: Cardiology and Cardiovascular Medicine

Cardiology and Cardiovascular MedicineMedicine