Acta Crystallographica Section D Structural Biology· 2019Q1
Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in <i>Phenix</i>
- 7,467citations
- Q1SCImago
- 2019year
Short summary
The Phenix software package has been updated to handle data from X-ray, neutron, and electron diffraction, as well as cryo-electron microscopy, for macromolecular structure determination.
AI-generated from the title and abstract; the full text is not read.
Key points
- Phenix software now integrates X-ray, neutron, electron diffraction, and cryo-EM data.
- Tools are customized for the unique properties of each experimental data type.
- Emphasis is placed on automating repetitive tasks in structure determination.
- A graphical user interface simplifies access to command-line features and project management.
AI-generated from the title and abstract; the full text is not read.
Abstract
Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
The authors' abstract, as published at the source. Acta Crystallographica Section D Structural Biology, 2019 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Materials Chemistry
Materials ChemistryMaterials Science