Journal of Agricultural and Food Chemistry· 2026Q1
A Novel Analytical Strategy to Detect Specific Markers of Nitrofurazone from Protein-Bound Metabolites in Food Animals
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- 2026year
Short summary
A novel analytical method using thiol reagents to substitute protein-bound nitrofurazone metabolites creates specific thiol-metabolite conjugates, enabling conclusive detection of nitrofurazone abuse in food animals.
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Key points
- Developed a novel analytical strategy to identify specific nitrofurazone markers.
- Used thiol reagents to substitute protein-bound nitrofurazone metabolites, forming specific thiol-metabolite conjugates.
- Confirmed marker structures using high-resolution mass spectrometry and stable isotopically labeled experiments.
- This method provides conclusive detection of nitrofurazone abuse, overcoming limitations of the nonspecific SEM marker.
AI-generated from the title and abstract; the full text is not read.
Abstract
Abstract Accurate detection of nitrofuran residues, as an indicator of banned drug use, in animal-derived food products is vital for ensuring food safety. The accepted regulatory approach for nitrofurans produces a residue marker from their protein-bound metabolites, which is nonspecific for nitrofurazone (i.e., semicarbazide (SEM)). Since SEM may originate from non-nitrofurazone sources, it is therefore not conclusive for nitrofurazone. Our main objective was to apply novel deconjugation/substitution reactions, which have been used to reverse protein binding, to identify a specific nitrofurazone marker. Bound nitrofurazone metabolites, produced using S9 from bovine liver or homogenized bovine liver tissue, were subjected to basic media and thiol reagents. Analyses carried out using high-resolution mass spectrometry showed that substitution of protein with thiol reagents produced thiol-metabolite conjugates whose structures were supported with stable isotopically labeled experiments. These conjugates can serve as new, specific markers for conclusive detection of nitrofurazone abuse in food animals.
The authors' abstract, as published at the source. Journal of Agricultural and Food Chemistry, 2026 · DOI ↗
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Field: Food Science
Food ScienceAgricultural and Biological Sciences