Kitosan ile stabilize edilmiş Ag–Ni çift metalik nanopartiküllerin kantitatif antibakteriyel değerlendirmesi ve potansiyel yara kaplama uygulamaları için

Özet (abstract)

Abstract Bimetallic silver–nickel nanoparticles (Ag–Ni BMNPs) stabilized with chitosan were synthesized and systematically characterized to evaluate their antibacterial properties and potential for proof-of-concept wound-coating applications. The synthesized nanoparticles were characterized using UV–Vis spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM), energy-dispersive X-ray spectroscopy (EDX), elemental mapping, dynamic light scattering (DLS), zeta-potential analysis, Brunauer–Emmett–Teller (BET) surface-area analysis, and thermogravimetric analysis (TGA). Additionally, X-ray photoelectron spectroscopy (XPS) confirmed the presence of Ag and Ni on the NP surface: mostly metallic Ag and both metallic Ni and Ni-oxide species. The NPs exhibited nanoscale morphology and moderate colloidal stability, with chitosan contributing to their stabilization. The antibacterial activity was tested against two Gram-positive ( Staphylococcus aureus and Bacillus subtilis ) and two Gram-negative ( Escherichia coli and Pseudomonas aeruginosa ) representative strains. Ag–Ni BMNPs exhibited considerably more antibacterial activity than the respective monometallic AgNPs and NiNPs based on zone of inhibition and minimum inhibitory concentration (MIC) analysis ( p < 0.05). For proof-of-concept coating applications, Ag–Ni BMNPs were incorporated into chitosan-based coatings and evaluated for antibacterial performance, coating integrity, and Ag + /Ni 2+ release using inductively coupled plasma optical emission spectroscopy (ICP-OES). The coatings exhibited in vitro antibacterial activity, showed no loss of physical integrity during PBS exposure, and showed time-dependent metal-ion release under the tested conditions. The results presented herein confirm the coating’s potential as a proof-of-concept platform and chitosan-stabilized Ag–Ni BMNPs as a promising material for antibacterial applications. However, further investigation of in vitro and in vivo cytotoxicity, hemocompatibility, biocompatibility, and wound-healing efficacy is needed before considering biomedical or clinical applications.

Yazarların özeti; kaynağından alınmıştır. Scientific Reports, 2026 · DOI ↗