Advanced Materials· 2026Q1
Microgels Enable iPSCs to Assemble, Expand, and Differentiate Into Organoids—From Sizable to High‐Throughput
- 2citations
- Q1SCImago
- 2026year
Short summary
Chemically defined PEG microgels, produced via microfluidics and coated with vitronectin, enable human induced pluripotent stem cells (iPSCs) to self-organize, expand, and differentiate into organoids, including cardiac and retinal tissues, compatible with 384-well plate automation.
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Key points
- Developed a chemically defined PEG microgel system for iPSC organoid generation.
- Microgels are produced via scalable microfluidics and coated with vitronectin for robust cell-material interaction.
- The system supports iPSC expansion, pluripotency maintenance, and differentiation into three germ layers.
- Demonstrated differentiation into cardiac organoids and retinal photoreceptors.
- The method is compatible with automated 384-well plate workflows for high-throughput applications.
AI-generated from the title and abstract; the full text is not read.
Abstract
Organoid research holds tremendous potential for personalized medicine and drug development. However, current limitations include reproducibility issues largely due to the use of biologically derived materials, which are prone to batch-to-batch variations. Here, we report a chemically defined microgel-based method for human induced pluripotent stem cell (iPSC)-based organoid generation, enabling expansion of iPSCs and their subsequent differentiation within one construct across different scales, including compatibility with automated 384-well plate workflows. Chemically defined poly(ethylene glycol) (PEG)-based microgels are produced via parallelized step-emulsification microfluidics, enabling scalable production. This approach leverages the self-organization of iPSCs with microgels to build three-dimensional constructs, driven by robust cell-material interactions achieved through vitronectin-coated PEG microgels. This technology allows the iPSCs to expand and retain their pluripotency, after which they can be differentiated into the three germ layers, providing a suitable platform for organoid differentiation. This was further extended by differentiation into cardiac organoids and retinal photoreceptors to demonstrate two exemplary tissues.
The authors' abstract, as published at the source. Advanced Materials, 2026 · DOI ↗
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Molecular MedicineBiochemistry, Genetics and Molecular Biology