Hu Mei Qing Yan Ke Li, Ağız Mikrobiyomunu Modüle Ederek Periodontiti Hafifletir

Özet (abstract)

BACKGROUND: Periodontitis is a prevalent chronic inflammatory disease driven by dysbiosis of the oral microbiota. Hu Mei Qing Yan Ke Li (HM), a modern herbal formulation derived from Reynoutria japonica Houtt and Prunus mume Siebold & Zucc, exhibits anti-inflammatory, antioxidant, and antibacterial properties, suggesting promising therapeutic potential for periodontitis therapy. This study aimed to investigate the protective effects of HM against experimental periodontitis and further explore its underlying mechanisms. METHODS: Periodontitis was induced by ligature placement in C57BL/6 mice. Mice were randomly assigned to the untreated periodontitis (P) and HM-treated (P + HM) groups. Mice in the P + HM group received HM via daily oral gavage at 6.58 mg/g body weight for 2 weeks. Alveolar bone loss was assessed by micro-CT analysis. Periodontal tissue destruction and osteoclast activity were evaluated by H&E, Masson's, and TRAP staining. Expression of OPG and RANKL was detected by immunofluorescence. Subgingival microbiota composition was analyzed by 16S rRNA sequencing. RESULTS: HM treatment significantly inhibited osteoclastogenesis and alleviated alveolar bone resorption, while preserving periodontal tissue integrity. Quantitatively, HM treatment significantly increased BV/TV from 41.89% to 54.41% (p = 0.0019), Tb.Th from 111.5 to 131.2 µm (p = 0.0028), and Tb.Sp from 119.2 to 153.6 µm (p = 0.0015), while decreasing BS/BV from 0.03166 to 0.02655 1/µm (p = 0.0004). Mechanistically, HM upregulated OPG and downregulated RANKL expression in periodontal tissues. Notably, HM restructured the subgingival microbial community, suppressing the pathobiont Streptococcus acidominimus (16.95%-9.37%, p = 0.0411) and Rodentibacter_A_734545pneumotropicus (70.32%-16.46%, p < 0.0001), while enriching a beneficial consortium including Streptococcus danieliae (0.75%-30.68%, p < 0.0001), unclassified Corynebacterium (0.00%-6.50%, p = 0.0022), and unclassified Ligilactobacillus (1.57%-22.74%, p = 0.0063). CONCLUSION: HM alleviates periodontitis by remodeling the dysbiotic oral microbiome toward homeostasis and favorably modulating the OPG/RANKL axis in a murine ligature-induced periodontitis model.

Yazarların özeti; kaynağından alınmıştır. Molecular Oral Microbiology, 2026 · DOI ↗