İnsanlarda Eşzamanlı Aerobik Egzersiz ile Enerji Açığının İskelet Kası Proteomik Yanıtları

Özet (abstract)

ABSTRACT Energy deficit is a potent physiological stressor that has shaped human evolution and can improve lifespan and healthspan in a wide range of species. Preserving locomotive capacity was likely essential for survival during the human hunter-gatherer period but surprisingly little is known about the molecular effects of energy deficit on human skeletal muscle, which is a key tissue for locomotion and metabolic health. Here we show that a 5-day 78% reduction in energy availability with concomitant aerobic exercise in healthy men leads to a profound modulation of skeletal muscle phenotype alongside increases in fat oxidation at rest and during exercise and a 2.1 ±0.8 kg loss of fat free mass and 0.8 ±0.6 kg of fat mass. We used stable isotope (D 2 O) labelling and peptide mass spectrometry to investigate the abundance and turnover rates of individual proteins. Abundance (1469 proteins) and synthesis rate (736 proteins) data discovered a shift toward a more oxidative phenotype and reorganisation of cytoskeleton and extracellular matrix structure during energy deficit. Mitochondrial components: TCA, electron transport chain and beta-oxidation, were prominently represented amongst proteins that increased in abundance and synthesis rate, as well as proteins related to mitochondrial proteostasis, remodelling and quality-control such as BDH1 and LONP1. Changes in muscle metabolic pathways occurred alongside a reduction in extracellular matrix proteins, which may counteract the age-related muscle fibrosis. Our results suggest that muscle metabolic pathways are not only preserved but positively affected during periods of concomitant low energy availability and exercise.

Yazarların özeti; kaynağından alınmıştır. bioRxiv (Cold Spring Harbor Laboratory), 2025 · DOI ↗