Journal of the Optical Society of America A· 2012Q2
Ptychographic transmission microscopy in three dimensions using a multi-slice approach
- 416citations
- Q2SCImago
- 2012year
Short summary
A new multi-slice ptychographic approach enables 3D imaging of thick samples, even with multiple scattering, overcoming limitations of traditional microscopy techniques.
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Key points
- Introduces a multi-slice ptychographic approach for 3D imaging.
- Successfully images samples with multiple scattering, unlike traditional methods.
- Overcomes the limitations of the projection approximation in tomography.
- Experimentally validated using visible light for thick sample imaging.
AI-generated from the title and abstract; the full text is not read.
Abstract
Generally, methods of three-dimensional imaging such as confocal microscopy and computed tomography rely on two essentials: multiple measurements (at a range of focus positions or rotations) and a weakly scattering specimen (to avoid distortion of the focal spot in the confocal microscope or to satisfy the projection approximation in tomography). Here we show that an alternative form of multi-measurement imaging, ptychography, can be extended to three dimensions and can successfully recover images in the presence of multiple scattering and when the projection approximation is not applicable. We demonstrate our technique experimentally using visible light, where it has applications in imaging thick samples such as biological tissues; however the results also have important implications for x ray and electron imaging.
The authors' abstract, as published at the source. Journal of the Optical Society of America A, 2012 · DOI ↗
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Field: Radiation
RadiationPhysics and Astronomy