iScience· 2026Q1
The RNA and protein landscapes of mouse brain organoids
- 1citations
- Q1SCImago
- 2026year
Short summary
Three-week-old mouse brain organoids closely mirror the transcriptome and proteome of neonatal mouse brains, including key neural identity markers like alternative splicing and synaptic proteins.
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Key points
- Three-week-old mouse brain organoids' transcriptome resembles neonatal mouse brains.
- Organoids reproduce the majority of alternative splicing and polyadenylation events defining neural identity.
- Proteomics shows most mRNA expression changes are translated into proteins.
- Organoids harbor a complex network of synaptic proteins, including glutamate and GABA receptors, after three weeks of differentiation.
AI-generated from the title and abstract; the full text is not read.
Abstract
Summary Organoids are powerful models of brain development and function. Mouse brain organoids reproduce the main steps of neurodevelopment in vivo and differentiate more rapidly than human organoids, making them scalable for screening applications. However, mouse brain organoids remain poorly defined. Here, we showed that the transcriptome of mouse brain organoids developed for three weeks is close to that of a neonatal mouse brain. Strikingly, organoids reproduced the majority of alternative splicing and polyadenylation site events that define a neural identity in vivo . Proteomics revealed that most changes in mRNA expression were translated into proteins. Mouse brain organoids differentiated for only three weeks already harboured a complex network of synaptic proteins, including the ionotropic and metabotropic receptors for the neurotransmitters glutamate and GABA. To conclude, our study provides compelling evidence that mouse brain organoids are a rapidly maturing and relevant model of mammalian brain development and function in a neonatal-like environment.
The authors' abstract, as published at the source. iScience, 2026 · DOI ↗
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Field: Developmental Neuroscience
Developmental NeuroscienceNeuroscience