PLoS ONE· 2012Q1
Mouse Estrous Cycle Identification Tool and Images
- 1,232citations
- Q1SCImago
- 2012year
Short summary
This paper presents a visual identification tool and a series of images to accurately determine the estrous cycle stage in mice, addressing challenges posed by coat color variations.
AI-generated from the title and abstract; the full text is not read.
Key points
- Visual identification of mouse estrous cycle stages is hindered by coat color differences.
- A series of images illustrates vaginal opening changes across all four estrous stages for black, agouti, and albino mouse strains.
- An identification tool is provided to assist users in determining estrous stage using vaginal cytology.
AI-generated from the title and abstract; the full text is not read.
Abstract
The efficiency of producing timed pregnant or pseudopregnant mice can be increased by identifying those in proestrus or estrus. Visual observation of the vagina is the quickest method, requires no special equipment, and is best used when only proestrus or estrus stages need to be identified. Strain to strain differences, especially in coat color can make it difficult to determine the stage of the estrous cycle accurately by visual observation. Presented here are a series of images of the vaginal opening at each stage of the estrous cycle for 3 mouse strains of different coat colors: black (C57BL/6J), agouti (CByB6F1/J) and albino (BALB/cByJ). When all 4 stages (proestrus, estrus, metestrus, and diestrus) need to be identified, vaginal cytology is regarded as the most accurate method. An identification tool is presented to aid the user in determining the stage of estrous when using vaginal cytology. These images and descriptions are an excellent resource for learning how to determine the stage of the estrous cycle by visual observation or vaginal cytology.
The authors' abstract, as published at the source. PLoS ONE, 2012 · DOI ↗
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Field: Agronomy and Crop Science
Agronomy and Crop ScienceAgricultural and Biological Sciences