Science· 2015Q1
Tissue-based map of the human proteome
- 16,073citations
- Q1SCImago
- 2015year
Short summary
Researchers have created a comprehensive map of the human tissue proteome, detailing protein presence and localization across 32 tissues and organs using an integrated omics approach.
AI-generated from the title and abstract; the full text is not read.
Key points
- Developed an integrated omics approach combining transcriptomics and immunohistochemistry for protein mapping.
- Mapped the proteome across 32 human tissues and organs, detecting over 90% of putative protein-coding genes.
- Enabled spatial localization of proteins down to the single-cell level.
- Explored specific proteomic aspects like the secretome, membrane proteome, and cancer proteome.
- Integrated all data into an interactive web-based database for public exploration.
AI-generated from the title and abstract; the full text is not read.
Abstract
Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray-based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body.
The authors' abstract, as published at the source. Science, 2015 · DOI ↗
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Field: Spectroscopy
SpectroscopyChemistry