Science· 2017Q1
A subcellular map of the human proteome
- 3,004citations
- Q1SCImago
- 2017year
Short summary
Researchers have created the Cell Atlas, a comprehensive image-based map detailing the subcellular location of 12,003 human proteins across 30 cellular structures, significantly advancing our understanding of cellular architecture.
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Key points
- Mapped the subcellular localization of 12,003 human proteins across 30 structures.
- Integrated transcriptomics, immunofluorescence microscopy, and mass spectrometry for comprehensive data.
- Defined proteomes for 13 major organelles.
- Discovered that approximately 50% of proteins exhibit abundance or localization in multiple cellular compartments.
- The Cell Atlas serves as a resource for refining protein-protein interaction networks.
AI-generated from the title and abstract; the full text is not read.
Abstract
Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.
The authors' abstract, as published at the source. Science, 2017 · DOI ↗
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Field: Spectroscopy
SpectroscopyChemistry