Cell· 2014Q1
Long-Term Culture of Genome-Stable Bipotent Stem Cells from Adult Human Liver
- 1,625citations
- Q1SCImago
- 2014year
Short summary
Researchers have developed a method to expand adult human liver stem cells long-term in culture, maintaining their genome stability and ability to differentiate into functional liver cells.
AI-generated from the title and abstract; the full text is not read.
Key points
- Adult human liver stem cells from bile ducts can be cultured long-term.
- Expanded cells maintain high chromosomal and structural stability.
- Cells can be differentiated into functional hepatocytes in vitro and in vivo.
- Patient-derived organoids model specific liver diseases like A1AT deficiency and Alagille syndrome.
AI-generated from the title and abstract; the full text is not read.
Abstract
Despite the enormous replication potential of the human liver, there are currently no culture systems available that sustain hepatocyte replication and/or function in vitro. We have shown previously that single mouse Lgr5+ liver stem cells can be expanded as epithelial organoids in vitro and can be differentiated into functional hepatocytes in vitro and in vivo. We now describe conditions allowing long-term expansion of adult bile duct-derived bipotent progenitor cells from human liver. The expanded cells are highly stable at the chromosome and structural level, while single base changes occur at very low rates. The cells can readily be converted into functional hepatocytes in vitro and upon transplantation in vivo. Organoids from α1-antitrypsin deficiency and Alagille syndrome patients mirror the in vivo pathology. Clonal long-term expansion of primary adult liver stem cells opens up experimental avenues for disease modeling, toxicology studies, regenerative medicine, and gene therapy.
The authors' abstract, as published at the source. Cell, 2014 · DOI ↗
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Field: Hepatology
HepatologyMedicine