Journal of Clinical Microbiology· 2014Q1
Bruker Biotyper Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry System for Identification of Nocardia, Rhodococcus, Kocuria, Gordonia, Tsukamurella, and Listeria Species
- 79citations
- Q1SCImago
- 2014year
Short summary
The Bruker Biotyper MALDI-TOF MS system achieved species-level identification for 89.7% of Listeria monocytogenes and 80% of Rhodococcus species, but struggled with Nocardia (14.9%), Kocuria (26.7%), Tsukamurella, and Gordonia.
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Key points
- Bruker Biotyper MALDI-TOF MS correctly identified 89.7% of Listeria monocytogenes and 80% of Rhodococcus species.
- Identification accuracy was lower for Nocardia (14.9%) and Kocuria (26.7%) species.
- The system failed to accurately identify Tsukamurella and Gordonia species.
- Clustering analysis revealed distinct spectral patterns for different Nocardia and Listeria monocytogenes clusters.
AI-generated from the title and abstract; the full text is not read.
Abstract
We evaluated whether the Bruker Biotyper matrix-associated laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) system provides accurate species-level identifications of 147 isolates of aerobically growing Gram-positive rods (GPRs). The bacterial isolates included Nocardia (n = 74), Listeria (n = 39), Kocuria (n = 15), Rhodococcus (n = 10), Gordonia (n = 7), and Tsukamurella (n = 2) species, which had all been identified by conventional methods, molecular methods, or both. In total, 89.7% of Listeria monocytogenes, 80% of Rhodococcus species, 26.7% of Kocuria species, and 14.9% of Nocardia species (n = 11, all N. nova and N. otitidiscaviarum) were correctly identified to the species level (score values, ≥ 2.0). A clustering analysis of spectra generated by the Bruker Biotyper identified six clusters of Nocardia species, i.e., cluster 1 (N. cyriacigeorgica), cluster 2 (N. brasiliensis), cluster 3 (N. farcinica), cluster 4 (N. puris), cluster 5 (N. asiatica), and cluster 6 (N. beijingensis), based on the six peaks generated by ClinProTools with the genetic algorithm, i.e., m/z 2,774.477 (cluster 1), m/z 5,389.792 (cluster 2), m/z 6,505.720 (cluster 3), m/z 5,428.795 (cluster 4), m/z 6,525.326 (cluster 5), and m/z 16,085.216 (cluster 6). Two clusters of L. monocytogenes spectra were also found according to the five peaks, i.e., m/z 5,594.85, m/z 6,184.39, and m/z 11,187.31, for cluster 1 (serotype 1/2a) and m/z 5,601.21 and m/z 11,199.33 for cluster 2 (serotypes 1/2b and 4b). The Bruker Biotyper system was unable to accurately identify Nocardia (except for N. nova and N. otitidiscaviarum), Tsukamurella, or Gordonia species. Continuous expansion of the MALDI-TOF MS databases to include more GPRs is necessary.
The authors' abstract, as published at the source. Journal of Clinical Microbiology, 2014 · DOI ↗
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Field: Microbiology
MicrobiologyMedicine