Cell· 2016Q1
Molecular Diversity of Midbrain Development in Mouse, Human, and Stem Cells
- 911citations
- Q1SCImago
- 2016year
Short summary
Single-cell RNA sequencing reveals 25 molecularly defined human ventral midbrain cell types, including radial glia and progenitors, and identifies conserved but distinct developmental timing and dopaminergic neuron diversification between humans and mice.
AI-generated from the title and abstract; the full text is not read.
Key points
- Identified 25 molecularly defined human ventral midbrain cell types, including five radial glia-like subtypes and four progenitor types.
- Observed diversification of two mature fetal dopaminergic neuron subtypes into five adult classes during postnatal development in mice.
- Found general conservation of cell types and gene expression between human and mouse midbrain development, with notable species-specific differences in timing and proliferation.
- Developed a quantitative, single-cell method to assess the fidelity of dopaminergic neurons derived from human pluripotent stem cells.
AI-generated from the title and abstract; the full text is not read.
Abstract
Understanding human embryonic ventral midbrain is of major interest for Parkinson's disease. However, the cell types, their gene expression dynamics, and their relationship to commonly used rodent models remain to be defined. We performed single-cell RNA sequencing to examine ventral midbrain development in human and mouse. We found 25 molecularly defined human cell types, including five subtypes of radial glia-like cells and four progenitors. In the mouse, two mature fetal dopaminergic neuron subtypes diversified into five adult classes during postnatal development. Cell types and gene expression were generally conserved across species, but with clear differences in cell proliferation, developmental timing, and dopaminergic neuron development. Additionally, we developed a method to quantitatively assess the fidelity of dopaminergic neurons derived from human pluripotent stem cells, at a single-cell level. Thus, our study provides insight into the molecular programs controlling human midbrain development and provides a foundation for the development of cell replacement therapies.
The authors' abstract, as published at the source. Cell, 2016 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Pediatrics, Perinatology and Child Health
Pediatrics, Perinatology and Child HealthMedicine