Clinical Efficacy and Safety Evaluation of a Centella asiatica (CICA)-Derived Extracellular Vesicle Formulation for Anti-Aging Skincare

Abstract

Centella asiatica (CICA)-derived exosomes have emerged as bioactive agents for skin rejuvenation due to their regenerative and anti-inflammatory properties. This study evaluated the safety and efficacy of a topical ampoule containing CICA-derived extracellular vesicles (EVs) in healthy Korean adults. This human application study was conducted over a 15-day period, during which the test formulation was topically applied to the skin following a controlled regimen. A 24-h patch test with 30 participants confirmed non-irritation (irritation index: 0.00). In a separate two-week trial (n = 20; mean age 50.7 years), 3D imaging and ultrasound assessed five-dimensional pore improvement (area, density, volume, filling, texture), wrinkle depth reduction in five facial regions, dermal hydration at 0.5, 1.5, and 2.5 mm depths, and skin density. Significant reductions were observed in mean pore area (−17.9%) and pore density (−26.9%), with a 9.0% decrease in surface roughness. Wrinkle depths decreased by 7.8–18.8% across the forehead, glabella, crow’s feet, nasolabial folds, and neck. Hydration increased by 7.9% at 0.5 mm, and dermal density improved by 12.7% (p < 0.05). These findings highlight the excellent skin compatibility and multifaceted cosmetic benefits of the formulation containing CICA-derived exosomes and other active ingredients, underscoring its potential as a safe, effective, and innovative anti-aging cosmetic agent.

The authors' abstract, as published at the source. Cosmetics, 2025 · DOI ↗