Journal of Proteome Research· 2026Q1
Implementation of an Internalized Hemolysis Assessment Approach in Targeted Bottom-Up Proteomics for the Purpose of Blood Plasma Sample Quality Control
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- Q1SCImago
- 2026year
Short summary
A new targeted proteomics method quantifies hemolysis markers to assess blood plasma sample quality, proposing inclusion in standard MRM analyses for reliable proteomic data.
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Key points
- Developed a targeted quantitative method to assess blood plasma sample quality by measuring hemolysis and coagulation markers.
- Created a scale to estimate the degree of hemolysis based on several protein markers.
- Investigated the feasibility of using an internal control approach to detect hemolysis in targeted proteomic analysis.
- Propose including plasma sample quality assessment and exclusion criteria into standard MRM analyses.
AI-generated from the title and abstract; the full text is not read.
Abstract
Abstract Mishandling of samples during blood plasma preparation may lead to biases in proteomic analysis and misinterpretation of data. An earlier study by Geyer et al. (2019) described proteins that may indicate poor plasma sample quality. Following this work, we have imitated a triangular strategy for biomarker discovery, established a targeted quantitative method for panels of hemolyzed and coagulated plasma sample markers proposed in the previous study, and validated it in our own model experiment. We have developed a scale for estimating the degree (rate) of hemolysis based on several protein markers and investigated the feasibility of using an internal control approach to detect hemolysis in targeted proteomic analysis. We propose including our plasma sample quality assessment and associated sample exclusion criteria into standard MRM analyses to provide more reliable data for proteomic research.
The authors' abstract, as published at the source. Journal of Proteome Research, 2026 · DOI ↗
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Field: Spectroscopy
SpectroscopyChemistry