Cell Reports· 2012Q1
A GAL4-Driver Line Resource for Drosophila Neurobiology
- 1,650citations
- Q1SCImago
- 2012year
Short summary
Researchers have created a resource of 7,000 transgenic fruit fly lines where GAL4 expression is driven by specific genomic DNA fragments, allowing precise control over gene expression in distinct neuronal subsets.
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Key points
- A collection of 7,000 transgenic Drosophila lines with GAL4 expression driven by defined genomic DNA enhancers.
- Expression patterns mapped in the adult brain and ventral nerve cord for 6,650 lines using confocal microscopy.
- Data allows for manual and machine-assisted annotation of neuronal expression.
- Demonstrates utility in identifying new neuronal cell types and analyzing brain asymmetry and neuronal stereotypy.
AI-generated from the title and abstract; the full text is not read.
Abstract
We established a collection of 7,000 transgenic lines of Drosophila melanogaster. Expression of GAL4 in each line is controlled by a different, defined fragment of genomic DNA that serves as a transcriptional enhancer. We used confocal microscopy of dissected nervous systems to determine the expression patterns driven by each fragment in the adult brain and ventral nerve cord. We present image data on 6,650 lines. Using both manual and machine-assisted annotation, we describe the expression patterns in the most useful lines. We illustrate the utility of these data for identifying novel neuronal cell types, revealing brain asymmetry, and describing the nature and extent of neuronal shape stereotypy. The GAL4 lines allow expression of exogenous genes in distinct, small subsets of the adult nervous system. The set of DNA fragments, each driving a documented expression pattern, will facilitate the generation of additional constructs for manipulating neuronal function.
The authors' abstract, as published at the source. Cell Reports, 2012 · DOI ↗
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Field: Cellular and Molecular Neuroscience
Cellular and Molecular NeuroscienceNeuroscience