Journal of Nanobiotechnology· 2026Q1
Engineered cryo-dead macrophages promote healing of MRSA-infected burn wounds via precise, long-acting photodynamic/antibiotic bacterial killing and broad-spectrum anti-inflammation
- 0citations
- Q1SCImago
- 2026year
Short summary
Engineered cryo-dead macrophages (Van@AIE-CD-M) loaded with vancomycin and a photosensitizer effectively treat MRSA-infected burn wounds by precisely killing bacteria and reducing inflammation.
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Key points
- Cryo-dead macrophages (CD-M) were engineered by freeze-killing live macrophages, preserving structure and receptors.
- Van@AIE-CD-M loaded with vancomycin and an aggregation-induced emission (AIE) photosensitizer target MRSA.
- The system enhances photodynamic antibacterial efficacy through MRSA binding to membrane-anchored photosensitizers.
- Engineered macrophages neutralize pro-inflammatory cytokines and release vancomycin over time.
- Treatment accelerated burn wound healing by reducing MRSA, inflammation, and promoting M2 polarization and angiogenesis.
AI-generated from the title and abstract; the full text is not read.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA)-infected burn wounds suffer from severe bacterial infection (resistant to almost all antibiotics) and intense inflammatory responses, leading to difficulty in wound healing. We designed a multifunctional cryogenic dead macrophage system (Van@AIE-CD-M) for precise, long-acting photodynamic/antibiotic MRSA eradication and broad-spectrum anti-inflammation. Cryo-dead macrophage (CD-M) carriers were prepared by freeze-killing live macrophages in liquid nitrogen, which eliminates the inflammatory side effects while preserving cellular structure and receptors. CD-M were then loaded with a high-performance aggregation-induced emission (AIE) photosensitizer TTVP on the membrane and vancomycin in the cytoplasm, yielding Van@AIE-CD-M. Following dripping onto MRSA-infected burn wounds, Van@AIE-CD-M utilized retained pattern-recognition receptors to bind MRSA, enhancing photodynamic antibacterial efficacy by bringing the bacteria close to the membrane-anchored photosensitizer. Meanwhile, pro-inflammatory cytokine receptors expressed on the Van@AIE-CD-M membrane neutralized multiple pro-inflammatory cytokines through specific receptor-ligand binding, achieving broad-spectrum anti-inflammatory effects. Moreover, Van@AIE-CD-M exhibited sustained vancomycin release, ensuring prolonged anti-infective efficacy. After treatment, Van@AIE-CD-M effectively reduced MRSA burden, pro-inflammatory cytokines, and macrophage M1 polarization, while notably increasing anti-inflammatory IL-10, macrophage M2 polarization, and angiogenesis, ultimately accelerating burn wound healing. This engineered CD-M offers a new strategy for treating burns complicated by drug-resistant bacterial infection and could be extended to other infectious diseases.
The authors' abstract, as published at the source. Journal of Nanobiotechnology, 2026 · DOI ↗
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Field: Rehabilitation
RehabilitationMedicine