Free Radical Research· 2026Q2
ABTS •+ radical cation-based new potentiometric approach for the studying of antioxidant capacity
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- 2026year
Short summary
A novel potentiometric method using the ABTS radical cation offers a stable, non-enzymatic approach to measure total antioxidant capacity, showing no significant difference from spectrophotometric methods and enabling analysis of colored samples like plant extracts and blood.
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Key points
- A new potentiometric approach for measuring total antioxidant capacity based on the ABTS radical cation is proposed.
- The method uses a stable cation radical/reduced form system generated non-enzymatically under specific conditions (1:0.5 ABTS:ammonium persulfate, 0.1 M KCl:ethanol 1:1, 24h at 25°C).
- The developed system remains stable in solid state for at least one month after drying at 70°C.
- Results from the potentiometric method showed no statistically significant differences compared to the classical spectrophotometric method.
- This method successfully analyzed colored samples (plant extracts, model whole blood) that are problematic for spectrophotometry.
AI-generated from the title and abstract; the full text is not read.
Abstract
The potentiometric approach for the studying of total antioxidant capacity, based on the use of the cation radical of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), is proposed. Conditions for non-enzymatic generation that enable the formation of a cation radical/reduced form system, subsequently employed as a potential-determining system have been established. The ratio of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) to ammonium persulfate was 1:0.5; the solvent was 0.1 M KCl and ethanol (1:1); generation (25 ± 2 °C) was carried out for 24 hours. It was shown that the system remains stable in the solid state for at least one month after drying at 70 °C. Individual antioxidants, plant extracts, and model biological fluids were investigated using the developed potentiometric approach. No statistically significant differences were observed between the results obtained by the classical spectrophotometric method and the proposed potentiometric method; the F- and t-tests did not exceed their critical values. Plant extracts with initial coloration and a model whole blood sample were investigated in the absorption range of the ABTS cation radical, which had previously been problematic using the spectrophotometry.
The authors' abstract, as published at the source. Free Radical Research, 2026 · DOI ↗
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Field: Organic Chemistry
Organic ChemistryChemistry