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Current Opinion in Genetics & Development· 2024Q1· Review

Beyond genomic weaving: molecular roles for CTCF outside cohesin loop extrusion

Aaron J. Corin, Elphège P. Nora, Vijay Ramani

Short summary

CTCF acts independently of cohesin to regulate nucleosome arrangement, chromatin accessibility, DNA replication, DNA repair, and RNA splicing, revealing multifaceted roles beyond its known function in 3D genome organization.

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Abstract

CCCTC-binding factor (CTCF) is a key regulator of 3D genome organization and transcriptional activity. Beyond its well-characterized role in facilitating cohesin-mediated loop extrusion, CTCF exhibits several cohesin-independent activities relevant to chromatin structure and various nuclear processes. These functions include patterning of nucleosome arrangement and chromatin accessibility through interactions with ATP-dependent chromatin remodelers. In addition to influencing transcription, DNA replication, and DNA repair in ways that are separable from its role in loop extrusion, CTCF also interacts with RNA and contributes to RNA splicing and condensation of transcriptional activators. Here, we review recent insight into cohesin-independent activities of CTCF, highlighting its multifaceted roles in chromatin biology and transcriptional regulation.

The authors' abstract, as published at the source. Current Opinion in Genetics & Development, 2024 · DOI ↗

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Molecular BiologyBiochemistry, Genetics and Molecular Biology