Nature Genetics· 2025Q1
Uniform dynamics of cohesin-mediated loop extrusion in living human cells
- 15citations
- Q1SCImago
- 2025year
Short summary
TAD anchors are brought together for 6-19 minutes about once per hour, driven by continuously extruding cohesin complexes, suggesting TAD dynamics are governed by CTCF site location and affinity.
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Key points
- TAD anchors are brought together for 6-19 minutes, approximately once per hour, in living human cells.
- TADs are continuously extruded by multiple cohesin complexes.
- Cohesin dynamics are consistent across diverse chromatin regions, irrespective of Hi-C pattern differences.
- TAD dynamics are primarily governed by the location and affinity of CTCF sites.
AI-generated from the title and abstract; the full text is not read.
Abstract
Most animal genomes are partitioned into topologically associating domains (TADs), created by cohesin-mediated loop extrusion and defined by convergently oriented CCCTC-binding factor (CTCF) sites. The dynamics of loop extrusion and its regulation remain poorly characterized in vivo. Here we tracked the motion of TAD anchors in living human cells to visualize and quantify cohesin-dependent loop extrusion across multiple endogenous genomic regions. We show that TADs are dynamic structures whose anchors are brought in proximity about once per hour and for 6-19 min (~16% of the time). Moreover, TADs are continuously extruded by multiple cohesin complexes. Remarkably, despite strong differences in Hi-C patterns across chromatin regions, their dynamics is consistent with the same density, residence time and speed of cohesin. Our results suggest that TAD dynamics is primarily governed by the location and affinity of CTCF sites, enabling genome-wide predictive models of cohesin-dependent chromatin interactions.
The authors' abstract, as published at the source. Nature Genetics, 2025 · DOI ↗
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Field: Molecular Biology
Molecular BiologyBiochemistry, Genetics and Molecular Biology