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Current Opinion in Genetics & Development· 2024Q1· Review

Attraction and disruption: how loop extrusion and compartmentalisation shape the nuclear genome

Mikhail Magnitov, Elzo de Wit

Short summary

Chromatin loops, key units of 3D genome organization, are shaped by loop extrusion (cohesin) and compartmentalisation (phase separation), which can interact to influence genome structure.

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Abstract

Chromatin loops, which bring two distal loci of the same chromosome into close physical proximity, are the ubiquitous units of the three-dimensional genome. Recent advances in understanding the spatial organisation of chromatin suggest that several distinct mechanisms control chromatin interactions, such as loop extrusion by cohesin complexes, compartmentalisation by phase separation, direct protein-protein interactions and others. Here, we review different types of chromatin loops and highlight the factors and processes involved in their regulation. We discuss how loop extrusion and compartmentalisation shape chromatin interactions and how these two processes can either positively or negatively influence each other.

The authors' abstract, as published at the source. Current Opinion in Genetics & Development, 2024 · DOI ↗

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Molecular BiologyBiochemistry, Genetics and Molecular Biology