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Journal of Visualized Experiments· 2025Q2

Toluidine Blue Staining of Peripheral Nerve Sections: A Technique to Stain Myelinated Nerve Fibers For Peripheral Nerve Structure Visualization

Short summary

A toluidine blue staining technique visualizes fine structures in resin-embedded, osmium tetroxide-treated rat peripheral nerve sections.

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Abstract

Source: Ghnenis, A. B. et al., Toluidine Blue Staining of Resin-Embedded Sections for Evaluation of Peripheral Nerve Morphology. J. Vis. Exp. (2018) This video demonstrates the staining technique of resin-embedded, osmium tetroxide-treated rat peripheral nerve sections using toluidine blue. The stained nerve sections help visualize the fine structures of peripheral nerves.

The authors' abstract, as published at the source. Journal of Visualized Experiments, 2025 · DOI ↗

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Field: Cellular and Molecular Neuroscience

Cellular and Molecular NeuroscienceNeuroscience