Büyük Başlı Sazan (Hypophthalmichthys nobilis) Kasında Düşük Sıcaklıkta Modifiye Atmosfer Taşıma Sırasında Mikrobiyal Topluluk Ardışıklığı ve Çevresel Tepkisi

Özet (abstract)

Microbial community succession contributes to quality changes in fresh freshwater fish during cold-chain transportation. In this study, bighead carp (Hypophthalmichthys nobilis) was used as the research material. Using 16S rRNA high-throughput sequencing, we characterized microbial community dynamics in fish muscle after 12 h and 24 h of simulated transportation under combined low-temperature (0 °C, 4 °C, 8 °C) and gradient CO2 modified atmosphere packaging (MAP) treatments. We further identified key environmental factors associated with shifts in bacterial communities during cold-chain logistics. The results showed that transportation temperature was the primary factor structuring microbial communities in bighead carp muscle, with stronger effects than MAP composition and transportation duration. Under 8 °C temperature conditions, low-to-moderate CO2 MAP corresponded with elevated microbial diversity and spoilage-associated succession, alongside enrichment of spoilage taxa including Pseudomonas. High-CO2 (80%) MAP altered community composition and reduced community complexity under low-temperature conditions, and the community changes exhibited a time-dependent pattern during extended transport. The combined treatment of 0 °C and 80% CO2 MAP generated distinct microbial succession profiles within 24 h and lowered the relative abundance of Pseudomonas. This study described the response patterns of bighead carp muscle microbiota to coupled low-temperature and MAP treatments and characterized community succession during cold-chain transport. The findings provide microbiota-based references for understanding microbial variation in freshwater fish cold-chain logistics.

Yazarların özeti; kaynağından alınmıştır. Foods, 2026 · DOI ↗