Société Française d'Ichtyologie· 2023
Revised procedures for staining and clearing small fishes and other vertebrates for bone and cartilage study
- 1,989citations
- 2023year
Short summary
A new protocol for staining bone and cartilage in small vertebrates minimizes damage by altering the dehydration and deacidification steps, improving preservation of delicate structures.
AI-generated from the title and abstract; the full text is not read.
Key points
- Specimens are dehydrated through alcohols before cartilage staining to minimize acetic acid activity on bone.
- Deacidification is performed directly in alkaline solutions after cartilage staining, avoiding lengthy alcohol transfers.
- The revised method aims to reduce loss or damage to bone and cartilage structures during processing.
- Variations are offered for staining bone alone, cartilage alone, or both simultaneously.
AI-generated from the title and abstract; the full text is not read.
Abstract
A revised procedure is described for counterstaining specimens with Alcian blue for cartilage and alizarin red S for bone, and for clearing tissue with enzymes and/or potassium hydroxide. Procedures are described and suggestions are made to minimize loss or damage to bone and cartilage structures during fixation, storage in collections, and clearing and staining specimens. This method differs from previously described procedures primarily in the following: specimens are not placed in water to remove formaldehyde following fixation. Instead they are dehydrated through a series of alcohols to minimize the acetic acid activity on bony structures while staining for cartilage. Following cartilage staining, specimens are immediately deacidified in alkaline solutions instead of transferring them through several series of alcohols. Suggested variations in the method are given for staining for bone alone, for cartilage alone or for both in the same specimen.
The authors' abstract, as published at the source. Société Française d'Ichtyologie, 2023 · DOI ↗
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Field: Rheumatology
RheumatologyMedicine