Science· 2013Q1
Oscillatory Control of Factors Determining Multipotency and Fate in Mouse Neural Progenitors
- 527citations
- Q1SCImago
- 2013year
Short summary
Oscillatory expression of key transcription factors (Ascl1, Hes1, Olig2) maintains multipotency in mouse neural progenitors, while sustained expression of a single factor drives differentiation into specific cell types.
AI-generated from the title and abstract; the full text is not read.
Key points
- Neural progenitor cells exhibit oscillatory expression of transcription factors Ascl1, Hes1, and Olig2.
- Oscillatory expression of these factors correlates with maintaining multipotency.
- Sustained expression of a single factor, such as Ascl1, drives differentiation into specific cell lineages.
- Optogenetics was used to manipulate Ascl1 expression and demonstrate its role in fate determination.
AI-generated from the title and abstract; the full text is not read.
Abstract
The basic helix-loop-helix transcription factors Ascl1/Mash1, Hes1, and Olig2 regulate fate choice of neurons, astrocytes, and oligodendrocytes, respectively. These same factors are coexpressed by neural progenitor cells. Here, we found by time-lapse imaging that these factors are expressed in an oscillatory manner by mouse neural progenitor cells. In each differentiation lineage, one of the factors becomes dominant. We used optogenetics to control expression of Ascl1 and found that, although sustained Ascl1 expression promotes neuronal fate determination, oscillatory Ascl1 expression maintains proliferating neural progenitor cells. Thus, the multipotent state correlates with oscillatory expression of several fate-determination factors, whereas the differentiated state correlates with sustained expression of a single factor.
The authors' abstract, as published at the source. Science, 2013 · DOI ↗
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Field: Developmental Neuroscience
Developmental NeuroscienceNeuroscience