Science· 2012Q1
Transcriptional Architecture and Chromatin Landscape of the Core Circadian Clock in Mammals
- 1,502citations
- Q1SCImago
- 2012year
Short summary
Mammalian circadian clocks exhibit a three-phase transcriptional cycle (poised, activation, repressed) involving CLOCK/BMAL1, with only 22% of cycling mRNAs driven by new transcription, suggesting significant post-transcriptional regulation.
AI-generated from the title and abstract; the full text is not read.
Key points
- Circadian clock transcription follows a three-phase cycle: poised, de novo activation, and repressed.
- CLOCK and BMAL1 are key activators in this loop.
- Only 22% of cycling mRNAs are driven by de novo transcription.
- Circadian regulation of RNA polymerase II recruitment and chromatin remodeling is genome-wide and extensive.
AI-generated from the title and abstract; the full text is not read.
Abstract
The mammalian circadian clock involves a transcriptional feed back loop in which CLOCK and BMAL1 activate the Period and Cryptochrome genes, which then feedback and repress their own transcription. We have interrogated the transcriptional architecture of the circadian transcriptional regulatory loop on a genome scale in mouse liver and find a stereotyped, time-dependent pattern of transcription factor binding, RNA polymerase II (RNAPII) recruitment, RNA expression, and chromatin states. We find that the circadian transcriptional cycle of the clock consists of three distinct phases: a poised state, a coordinated de novo transcriptional activation state, and a repressed state. Only 22% of messenger RNA (mRNA) cycling genes are driven by de novo transcription, suggesting that both transcriptional and posttranscriptional mechanisms underlie the mammalian circadian clock. We also find that circadian modulation of RNAPII recruitment and chromatin remodeling occurs on a genome-wide scale far greater than that seen previously by gene expression profiling.
The authors' abstract, as published at the source. Science, 2012 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Endocrine and Autonomic Systems
Endocrine and Autonomic SystemsNeuroscience