High-resolution transcriptomic and proteomic profiling of house dust mite homologous allergens

Abstract

The dataset describes the transcriptome and proteome of Dermatophagoides pteronyssinus and D. farinae, focusing on allergen production. The scripts.zip contains Python and R scripts with a description in Readme.md. The FASTA files include Dermatophagoides farinae and D. pteronyssinus homologous allergen proteins and cDNA, and proteins rejected during the analyses. The original data included feces/bodies proteomes and gene expression for predicted transcripts. The next files included the steps of homologous allergen identification and quantification based on proteome and expression data. CSV files inventory (inventory.txt) DF_allergen_group.csv; the list of homologous allergens pooled per group in the samples of Dermatophagoides farinaeDF_allergen_profile.csv; the list of allergens with homologous transcript variants in the samples of Dermatophagoides farinaeDF_DP_allergen_list.csv; the list of allergens with homologous transcript variants in the samples of Dermatophagoides farinae and D. pteronyssinusDF_DP_hmmer_pairwise_candidates.csv; the list of 187 identified homologous allergens with transcript variants in both mitesDF_DP_proportion.csv; the proportion of the groups of homologous allergens of both mites inside the compartments - not standardized dataDF_DP_proteome_only_allergens.csv; the list of allergens identified in the proteome without homologous transcript variantsDF_DP_rejected_low_transcript.csv; the list of allergens with homologous transcript variants without expression among them; those without proteome identification were removedDF_expression.csv; the unstandardized expression of homologous transcript variants in Dermatophagoides farinaeDF_proteome_bodies.csv; proteome data for Dermatophagoides farinae bodies, standardizedDF_proteome_feces.csv; proteome data for Dermatophagoides farinae feces, standardizedDP_allergen_group.csv; the list of homologous allergens pooled per group in the samples of Dermatophagoides pteronyssinusDP_allergen_profile.csv; the list of allergens with homologous transcript variants in the samples of Dermatophagoides pteronyssinusDP_expression.csv; the unstandardized expression of homologous transcript variants in Dermatophagoides pteronyssinusDP_proteome_bodies.csv; proteome data for Dermatophagoides pteronyssinus bodies, standardizedDP_proteome_feces.csv; proteome data for Dermatophagoides pteronyssinus feces, standardizedHDM_comparison.csv; homologous transcript variants identification according to curated datasets including protein descriptionHDM_edited.csv; homologous transcript variants identification according to curated datasets including protein description, with sequences identified outside protein alignments to the curated databasePooled_allergen.csv; list of allergen samples pooled into allergen groups and their intensity in proteome or expressionspecies_comparison_all.csv; comparison of intensity or expression of homologous transcript variants in proteome according to sex and extraction methodspecies_comparison.csv; pooled allergen group comparison between species for compartments using limma FDRspecies_comparison_body_feces.csv; pooled allergen group comparison between species for extraction method in the feces using limma FDRspecies_comparison_proteome.csv; pooled allergen group comparison between species for sex in the bodies using limma FDR

The authors' abstract, as published at the source. International Immunopharmacology, 2026 · DOI ↗