Journal of Agricultural and Food Chemistry· 2026Q1
LC-MS/MS Quantification of Total Milk Protein Allergens: A Dual-Target Approach Using β-Lactoglobulin/β-Casein Standards and Stable Isotope-Labeled Peptide Calibration
- 0citations
- Q1SCImago
- 2026year
Short summary
A new LC-MS/MS method accurately quantifies total milk allergens in processed foods by simultaneously measuring whey (β-lactoglobulin) and casein (β-casein) fractions using specific marker peptides and stable isotope-labeled internal standards.
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Key points
- Developed a dual-target LC-MS/MS method to quantify both whey (β-lactoglobulin) and casein (β-casein) milk protein fractions simultaneously.
- Utilizes specific marker peptides (LIVTQTMK for β-lactoglobulin, VLPVPQK for β-casein) and stable isotope-labeled peptide internal standards.
- The method accurately determines total milk allergens even with processing-induced changes in whey-to-casein ratios.
- Achieved detection limits of 0.1 mg/kg, recovery rates of 80.6%–92.4%, and precision below 12.7% RSD.
- Provides a robust analytical tool for allergen control and regulatory compliance in processed foods.
AI-generated from the title and abstract; the full text is not read.
Abstract
Abstract Accurate quantification of total milk protein allergens in foods remains challenging due to processing-induced alterations in whey-to-casein ratios and matrix interference effects. Herein, a LC-MS/MS method was developed and validated for accurate quantification of milk allergens in foods using a novel dual-target approach. This method simultaneously quantifies whey and casein fractions using high-purity protein standards of β-lactoglobulin and β-casein through marker peptides LIVTQTMK and VLPVPQK, respectively, coupled with stable isotope-labeled peptide internal standards containing flanking sequences. The dual-target strategy overcomes limitations of conventional single-target methods by providing accurate total milk protein determination regardless of processing-induced alterations in protein ratios. Method validation demonstrated excellent performance with detection limits of 0.1 mg/kg for β-lactoglobulin and β-casein, recovery rates of 80.6%–92.4%, and precision below 12.7% RSD. This dual-target approach provides a robust analytical tool for accurate milk allergens determination in processed foods, significantly advancing allergen control and regulatory compliance.
The authors' abstract, as published at the source. Journal of Agricultural and Food Chemistry, 2026 · DOI ↗
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Field: Immunology and Allergy
Immunology and AllergyMedicine