Scientific Reports· 2020Q1
Re-epithelialization and immune cell behaviour in an ex vivo human skin model
- 8,289citations
- Q1SCImago
- 2020year
Short summary
Researchers developed a novel ex vivo human skin wound model using negative pressure, which successfully recapitulates epidermal regeneration and immune cell dynamics observed in natural wound healing.
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Key points
- A novel ex vivo human skin wound model was created using negative pressure, preserving the basement membrane.
- The model demonstrated keratinocyte proliferation, migration, and epidermal differentiation within 6-12 days.
- Increased T cells and unique immune cell populations were observed in the wounded skin compared to normal skin.
- The model recapitulates key features of human wound healing and can be used for therapeutic testing.
AI-generated from the title and abstract; the full text is not read.
Abstract
Abstract A large body of literature is available on wound healing in humans. Nonetheless, a standardized ex vivo wound model without disruption of the dermal compartment has not been put forward with compelling justification. Here, we present a novel wound model based on application of negative pressure and its effects for epidermal regeneration and immune cell behaviour. Importantly, the basement membrane remained intact after blister roof removal and keratinocytes were absent in the wounded area. Upon six days of culture, the wound was covered with one to three-cell thick K14 + Ki67 + keratinocyte layers, indicating that proliferation and migration were involved in wound closure. After eight to twelve days, a multi-layered epidermis was formed expressing epidermal differentiation markers (K10, filaggrin, DSG-1, CDSN). Investigations about immune cell-specific manners revealed more T cells in the blister roof epidermis compared to normal epidermis. We identified several cell populations in blister roof epidermis and suction blister fluid that are absent in normal epidermis which correlated with their decrease in the dermis, indicating a dermal efflux upon negative pressure. Together, our model recapitulates the main features of epithelial wound regeneration, and can be applied for testing wound healing therapies and investigating underlying mechanisms.
The authors' abstract, as published at the source. Scientific Reports, 2020 · DOI ↗
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Field: Rehabilitation
RehabilitationMedicine