eLife· 2016Q1
Sensitive red protein calcium indicators for imaging neural activity
- 1,145citations
- Q1SCImago
- 2016year
Short summary
New red genetically encoded calcium indicators (jRCaMP1a, b and jRGECO1a) achieve sensitivity comparable to state-of-the-art green indicators (GCaMP6), enabling deep-tissue imaging and dual-color experiments.
AI-generated from the title and abstract; the full text is not read.
Key points
- Introduced new red GECIs (jRCaMP1a, b and jRGECO1a) with sensitivity comparable to GCaMP6.
- Validated performance in cultured neurons and in vivo across mouse, Drosophila, zebrafish, and C. elegans.
- Red-shifted spectra reduce tissue scattering, absorption, and phototoxicity compared to green GECIs.
- Enables deep-tissue imaging and dual-color imaging with GFP-based reporters.
AI-generated from the title and abstract; the full text is not read.
Abstract
Genetically encoded calcium indicators (GECIs) allow measurement of activity in large populations of neurons and in small neuronal compartments, over times of milliseconds to months. Although GFP-based GECIs are widely used for in vivo neurophysiology, GECIs with red-shifted excitation and emission spectra have advantages for in vivo imaging because of reduced scattering and absorption in tissue, and a consequent reduction in phototoxicity. However, current red GECIs are inferior to the state-of-the-art GFP-based GCaMP6 indicators for detecting and quantifying neural activity. Here we present improved red GECIs based on mRuby (jRCaMP1a, b) and mApple (jRGECO1a), with sensitivity comparable to GCaMP6. We characterized the performance of the new red GECIs in cultured neurons and in mouse, Drosophila, zebrafish and C. elegans in vivo. Red GECIs facilitate deep-tissue imaging, dual-color imaging together with GFP-based reporters, and the use of optogenetics in combination with calcium imaging.
The authors' abstract, as published at the source. eLife, 2016 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Cellular and Molecular Neuroscience
Cellular and Molecular NeuroscienceNeuroscience