STAR Protocols· 2026Q2
Protocol for generating highly enriched sex-specific primary cortical astrocytes from individual mouse pups
- 0citations
- Q2SCImago
- 2026year
Short summary
A new protocol enables the generation of highly enriched, sex-specific primary cortical astrocytes from individual neonatal mouse pups, allowing for sex- and genotype-defined cell cultures.
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Key points
- Protocol for generating highly enriched primary cortical astrocytes from individual neonatal mouse pups.
- Includes mechanical dissociation and culturing in coated flasks.
- Removes oligodendrocyte progenitor cells via mechanical shaking.
- Depletes microglia using selective pharmacological agents.
- Utilizes PCR-based analysis of tail-biopsy DNA for sex and genotype determination.
AI-generated from the title and abstract; the full text is not read.
Abstract
Here, we present a protocol for generating highly enriched primary cortical astrocytes from individual neonatal mouse pups. We describe steps for mechanically dissociating cortical tissue, culturing in coated flasks, and removing oligodendrocyte progenitor cells through mechanical shaking and selective pharmacological depletion of microglia. We then detail procedures for PCR-based analysis of tail-biopsy DNA to determine sex and genotype to genetically define astrocyte cultures. This protocol enables the investigation of sex- and genotype-specific mechanisms while avoiding the confounding effects of pooled tissue.
The authors' abstract, as published at the source. STAR Protocols, 2026 · DOI ↗
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Field: Developmental Neuroscience
Developmental NeuroscienceNeuroscience