Journal of Berry Research· 2026Q2
Tissue-specific anthocyanin quantification, comprehensive phytochemical profiling, and chemometric analysis of fruit and aerial parts of Rubus buergeri Miq.
- 0citations
- Q2SCImago
- 2026year
Short summary
A new UHPLC-PDA method quantifies anthocyanins in Rubus buergeri, revealing distinct tissue-specific profiles: aerial parts are rich in cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside, while fruits show higher cyanidin and pelargonidin levels after hydrolysis.
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Key points
- Developed and validated a UHPLC-PDA method for simultaneous quantification of anthocyanins and anthocyanidins.
- Quantified cyanidin 3-O-glucoside and cyanidin 3-O-rutinoside in aerial parts and fruits, noting higher C3R in fruits.
- After acid hydrolysis, cyanidin was more abundant in aerial tissue, while fruits had higher cyanidin and significantly more pelargonidin.
- Identified 137 metabolites using LC-QToF-MS and used chemometrics to show distinct metabolic clustering between tissues.
AI-generated from the title and abstract; the full text is not read.
Abstract
Rubus buergeri , a winter-fruiting species native to East Asia, remains poorly characterized phytochemically. This study presents the first comprehensive phytochemical profiling of fruits and aerial parts collected from Jeju Island, Korea. A validated ultra-high-performance liquid chromatography–photodiode array (UHPLC–PDA) method enabled simultaneous quantification of anthocyanins and anthocyanidins. Quantitative analysis revealed pronounced tissue-specific and inter-individual variation. Aerial tissues contained cyanidin 3- O -glucoside (C3G; 9.1–125.0 μg/g) and cyanidin 3- O -rutinoside (C3R; 7.9–51.1 μg/g), while fruits showed relatively higher C3R levels (14.5–58.2 μg/g). Following acid hydrolysis, cyanidin was generally more abundant in aerial tissue (1298–3487 µg/g), whereas fruits contained cyanidin (1035–3563 µg/g) and markedly higher pelargonidin (279.7–555.5 µg/g). LC–QToF-MS identified 137 metabolites, and chemometric analyses revealed clear metabolic clustering between tissues. This integrative approach provides a robust dataset for understanding phytochemical composition, tissue-specific metabolic specialization, and potential functional properties of R. buergeri .
The authors' abstract, as published at the source. Journal of Berry Research, 2026 · DOI ↗
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Field: Biochemistry (Medicine)
BiochemistryMedicine