Cell· 2018Q1
A Complete Electron Microscopy Volume of the Brain of Adult Drosophila melanogaster
- 1,166citations
- Q1SCImago
- 2018year
Short summary
Researchers have created the first complete electron microscopy (EM) volume of an adult fruit fly brain at synaptic resolution, enabling comprehensive neural circuit mapping.
AI-generated from the title and abstract; the full text is not read.
Key points
- The complete brain of an adult female Drosophila melanogaster was imaged using electron microscopy at synaptic resolution.
- A custom high-throughput EM platform was developed to overcome the size limitations of conventional EM for the fly brain.
- Mapping of mushroom body circuitry revealed a new cell type and unexpected organization of olfactory projection neurons.
- The dataset provides a comprehensive resource for mapping brain-spanning neural circuits in Drosophila.
AI-generated from the title and abstract; the full text is not read.
Abstract
Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only electron microscopy (EM) enables complete, unbiased mapping of synaptic connectivity; however, the fly brain is too large for conventional EM. We developed a custom high-throughput EM platform and imaged the entire brain of an adult female fly at synaptic resolution. To validate the dataset, we traced brain-spanning circuitry involving the mushroom body (MB), which has been extensively studied for its role in learning. All inputs to Kenyon cells (KCs), the intrinsic neurons of the MB, were mapped, revealing a previously unknown cell type, postsynaptic partners of KC dendrites, and unexpected clustering of olfactory projection neurons. These reconstructions show that this freely available EM volume supports mapping of brain-spanning circuits, which will significantly accelerate Drosophila neuroscience. VIDEO ABSTRACT.
The authors' abstract, as published at the source. Cell, 2018 · DOI ↗
Continue with a free account
Ask the paper: 3 free questions a day about this paper; save it, get its citation, new summaries every day for your field. Takeaways are Premium.
Continue free on the webSign in with Google or Apple; no card needed. You come back to this paper.
On your phone:
Field: Cellular and Molecular Neuroscience
Cellular and Molecular NeuroscienceNeuroscience